Extraction of Chlorogenic Acid
This user is a laboratory in France, whose main purpose is to extract chlorogenic acid from honeysuckle.
Chlorogenic acid is an organic compound with the chemical formula C16H18O9. It is one of the main antibacterial and antiviral active ingredients of honeysuckle. The hemihydrate is needle-shaped crystals (water). It turns into anhydrous compound at 110℃. Its solubility in water at 25℃ is 4%, and it is more soluble in hot water. It is easily soluble in ethanol and acetone, and is extremely insoluble in ethyl acetate.
Detailed Explanation of the Entire Process of Extracting Chlorogenic Acid
Sample Preparation and Sampling: Select plant samples rich in chlorogenic acid, such as honeysuckle and eucommia leaves, and take fresh samples for sampling.
Pre-treatment: The samples are subjected to cleaning, cutting, and crushing processes to increase the surface area, facilitating the extraction of chlorogenic acid.
Refrigeration preservation: Place the processed samples in a refrigerated environment below 4 degrees Celsius for storage, to prevent decomposition and degradation.
Extract: Select appropriate organic solvents, such as ethanol and methanol, and use a homogenizer to perform cell disruption treatment to ensure effective combination of the cells with the solvents.
Filtering: At room temperature, the residue and liquid are quickly separated using a filter press.
Concentration: The crude extract is further concentrated using methods such as vacuum concentration apparatus to increase the concentration of chlorogenic acid.
Purification: The chlorogenic acid aqueous extract is filtered through an ultrafiltration membrane to remove large molecules such as polysaccharides and proteins, ensuring the purity.
Decolorization and Filtration: Use adsorbents such as activated carbon to remove pigments and impurities from the solution, and then perform filtration to remove solid impurities and residual activated carbon.
Drying and Storage: The purified chlorogenic acid solution should undergo drying treatment, such as spray drying or vacuum drying, while controlling the temperature and time to prevent loss and degradation. The dried powder should be stored in a dry, cool, and well-ventilated place, avoiding direct sunlight and high temperatures. Pay attention to moisture prevention and pest control.
At each step, the operating conditions and parameters need to be strictly controlled to ensure the extraction efficiency and the purity and quality of chlorogenic acid.
